SAMUEL DWI WARDIYANTO, 050911209 (2014) STUDI POTENSI AKTIVITAS ANTI RADIKAL BEBAS EKSTRAK ETANOL 80% Mitrephora polypyrena (Bl.) Miq., Lepisanthes rubiginosum (Roxb.) Leenh. dan Alectryon serratus Radlk DENGAN METODE KLT AUTOGRAFI DAN SPEKTROFOTOMETRI. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The extracts were placed on TLC plate and eluated by ethyl acetate : chloroform : methanol: formic acid (5:3:2:1). Then TLC plates sprayed with 0,2 % DPPH in methanol. The presence of purple DPPH bleaching on TLC plate was meant to show existence of free radical scavenging activity. The instrument for spectrophotometry method is UV-Vis spectrophotometer. Absorbance of the extracts were measured at three visible wavelengths ( 497 nm,517 nm, and 537 nm) by reacting 0,3 mL sampel solution (various concentration) with 2,7 mL 0,004% DPPH in methanol solution. The IC50 value of each extracts was analyzed by using linear regression and compared with the IC50 value of ascorbic acid (vitamin C) as standards. The result showed that the 80% ethanol extract of Alectryon serratus leaf had the smallest IC50 with the value was 1,964 ppm, which means have the highest free radical scavenging activity among the other extracts. Contrarily, the 80% ethanol extract of Mitrephora polypyrena leaf had the lowest free radical scavenging activity among the other extracts with the largest IC50 was 93,48 ppm

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