HANIFAH AYU PRAMITASARI, 021011130 (2013) UJI SITOKSISITAS EKSTRAK DAUN SIRIH MERAH (PIPER CROCATUM) TERHADAP SEL FIBROBLAS BHK 21. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background: Red betel leaf extract (Piper Crocatum) is known to be a root canal irrigation alternative medicine because it has antibacterial effects and can dissolve the smear layer on the root canal.The red betel leaf contain saponin, tannin and flavanoids but saponin in high concentration can be toxic to human cells. Purpose: The aim of this study was to identify the effects of red betel leaf extract (Piper Crocatum) cytoxicity in BHK 21 fibroblast cell with MTT assay. Method. The sample was Baby Hamster Kidney fibroblast cell (BHK 21). The red betel leaf extract (Piper Crocatum) divide into 5 concentration (100%, 50%, 25%, 12,5%, 6,25%). These extract applied into fibroblast cell culture BHK 21 and incubated in 24 hours. After incubated ELISA Reader used to read the result of this experiment. Result. The study shows that Red betel leaf (Piper Crocatum) is non toxic in BHK 21 fibroblast cell. From the study also showed that increasing of fibroblast proliferation happened when Red betel leaf extract applied to the culture. Conclusion. Red betel leaf (Piper Crocatum) extract with concentration 100%, 50%, 25%, 12,5%, 6,25% are non toxic use in BHK 21 fibroblast cell.

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