RIZKI PURNAMASARI NUGRAHENI, 020710149 (2010) UJI SENSITIVITAS ANTIBODI MONOKLONAL Streptococcus mutans 1(c) 67 kDa (SENSITIVITY TEST OF MONOCLONAL ANTIBODIES Streptococcus mutans 1(c) 67 kDa). Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background. Based on SKRT 2004, dental caries prevalence rate in Indonesia reached 90,05%. Local passive immunization with monoclonal antibodies is a new alternative in caries prevention. Monoclonal antibodies S.mutans are specific antibodies against epitope antigen S.mutans 67 kDa protein. Antibody and antigen bonding will inactivate epitope antigen so that the attachment of S.mutans can be inhibited and the number of colonies decreased. Monoclonal antibodies used in this research is a monoclonal antibody S.mutans 1 serotype (c) 67 kDa subclass IgA, IgG1 and IgG3 that have been made in Pusvetma, Surabaya. Purpose. The aim of this research is to determine whether monoclonal antibodies sensitivity against epitope antigen S.mutans derived from stock culture and ATCC MT8148 serotype c. Method. The sensitivity test of monoclonal antibodies S.mutans used immunodiffusion technique Ouchterlony (IDT) and single radial immunodiffusion technique (RID). The measurement sensitivity of monoclonal antibodies when there is a line or ring precipitation in agarose plate by IDT and RID technique. Result. There was no line or ring precipitation in IDT and RID test. This condition may be caused by several things that can damage protein molecule of monoclonal antibodies, one of the storage method is less precise. Conclusion. Monoclonal antibodies S.mutans 1 serotype (c) 67 kDa subclass IgA, IgG1 and IgG3 have no possess the power of sensitivity in identifying epitope antigen of S.mutans.

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