Yessica Nainggolan, 020513583 (2010) KONSENTRASI HAMBAT MINIMAL BAHAN INFUSUM RIMPANG LENGKUAS (Alpinia galanga Linn.) TERHADAP PERTUMBUHAN Streptococcus viridans. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background. Dental caries is a dental disease that most often found. Microorganisms that cause dental caries activity on the outer surface of the teeth may spread into the pulp and root canal then cause root canal infection. The study found the dominance of bacteria Streptococcus viridans in root canal infections. Root canal infection needs root canal treatment. The use of proper medikamen root canal will support the success of root canal treatment. Galangal is one of medicinal plants that contain the diversity of chemical structures potent anti bacterial, anti fungal and anti inflammatory. Purpose. The aim of this study was to evaluate an anti bacterial effect and to determine the minimal inhibitory concentration of galangal rhizome (Alpinia galanga Linn.) infusion againts the growth of Streptococcus viridans. Method. Research carried out by using serial dilution methods, that is mixed colony suspension Streptococcus viridans in BHIB media (Brain Heart Infusion Brothers) and infusion materials, then were incubated for 24 hours at 370C. There are 4 research groups, the concentration of 25%, 20%, 15%, and 12.5%. The observation of growth of bacteria colonies is done by visually comparing the turbidity, and then made bacterial colonies growing on blood agar media. Growth of bacterial colonies in blood agar is calculated manually in colony forming unit (cfu). Result. At the concentration of 25%, 20%, 15%, and 12.5%, there are a decrease in the number of Streptococcus viridans bacterial colonies when compared with positive control group. There are significant differences in each study group (p <0.05). At the concentration of 25%, we obtained least of bacterial colonies and the number of bacterial colonies most numerous in the concentration of 12.5%. Conclusion. The minimal inhibitory concentration infusion materials galangal rhizome (Alpinia galanga Linn.) in inhibiting the growth of Streptococcus viridans is 25%.

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