SITI ELIANA ROCHMI, 060911123 (2013) PASASE BERSERI PRECOCIOUS LINES SEBAGAI UPAYA ATENUASI PATOGENITAS Eimeria tenella DITINJAU DARI PERKEMBANGAN MORFOLOGI DAN MORFOMETRI STADIUM SEKSUAL. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of the research was to produce an attenuated strain of Eimeria tenella by the serial precocious lines. This research used the morphometry (cross sectional area / size) of sporulated oocyst, time and percentage of sporulation, and oocyst production that the E. tenella had been attenuated. Four parts series has occurred in 24 broilers with five male chicken for treatments and one male chicken for control each passage. First part used E. tenella parent lines, the second part of the precocious lines used E. tenella of the first part, the third part of the precocious lines E. tenella used of the second part, then the fourth part of the precocious lines E. tenella used of the third part. the sample used a feces was processed by sediment and floating method. The oocysts obtained was sporulated by potassium bichromate 2.5% in room temperature. The morphometry of sporulated oocyst was measured by Moticplus 2.0 program with magnification 400x. The oocyst production was counted by Universal Whitlock McMaster Chamber modification under microscope with magnification 100x. The result showed there was significantly different (p<0.05) in the time of percentage 100% sporulation between the first, second, and third passage. The fourth passage was the same as the third passage. The morphometry of sporulated oocyst showed significantly different (p<0.05) between the first, second, third, and fourth passage. The oocyst production showed significantly different (p<0.05) between the first, second, third, and fourth passage.

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