RENDY SURYAADY PRATAMA, 061011232 (2014) PENGARUH PENAMBAHAN HY ANTISERA DALAM PENGENCER SKIM KUNING TELUR TERHADAP PERSENTASE VIABILITAS SPERMATOZOA X DAN Y PADA SEMEN SAPI SIMENTAL. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This research aimed to determine the percentage viability of X and Y spermatozoa after treated by addition HY antisera on egg yolk skim diluent on Simental Cattle semen. This study was used eight samples, four samples as a control and four samples treated by HY antisera with incubation time 15, 30, 45 and 60 minutes at 37oC. Spermatozoa preparation were stained with eosinnegrosin then examined by “optilab viewer” to count the viability of spermatozoa, length and width of head spermatozoa. Result of X spermatozoa was not significant difference (P>0.05) with the best percentage viability X spermatozoa in control and treated HY antisera in 15 minutes. Y spermatozoa showed significant difference (P<0.05) with the best percentage viability Y spermatozoa in control in 15 minutes. HY antisera addition had no effect on the percentage viability of X spermatozoa but had effect decreased on percentage viability of Y spermatozoa.

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