SRUTI LISTRA ADRENALIN, 061011092 (2014) ANTIGENISITAS PROTEIN Ascaridia galli TERHADAP SERUM MENCIT Anti-Ascaridia galli DAN Anti-Raillietina echinobothrida DENGAN METODE ELISA. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this research was to find out cross reaction between antigen A._galli with anti-R. echinobothrida sera by using indirect-ELISA method. This research was conducted by introducing protein with whole worm extract (WWE) A. galli. The first step, A. galli and R._echinobothrida that was collected from poultry intestine, produced and homogenized by doing sonication and sentrifuge to obtain the homogenates. The second step, immunization of each mice group with adjuvant (P0), homogenates A. galli (P1), homogenates R._echinobothrida (P2). The third step, optical density (OD) value was determined by indirect-ELISA method. The result showed that 1) mean OD value control antibody was 0,084, anti-A. galli sera mice was 0,335 and anti-R._echinobothrida sera mice was 0,227; 2) The analisis statistic was very significant differences between P0, P1, P2 and there was cross reaction between antigen A._galli with antibody R._echinobothrida; 3) sensitivity was 100%, specificity was 12,5%, and false positif was 87,5%.

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