NADYA PERMATA, 061011269 (2014) ANTIGENISITAS PROTEIN Ascaridia galli TERHADAP SERUM Raillietina tetragona MENGGUNAKAN TEKNIK ELISA. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this study was to find out the antigenicity of Ascaridia galliprotein against anti-Raillietina tetragona using indirect-ELISA technique. The first phase, Ascaridia galli and Raillietina tetragona worms were collected and identified, then preparing homogenate by Whole Worm Extract (WWE) of Ascaridia galli and Raillietina tetragona worms and measuringthe protein content of the Ascaridia galli worm. The second phase, preparation of polyclonal antibody of Ascaridia galli and Raillietina tetragona were obtained from homogenate immunizated mice. The third phase, determination of OD value usedindirect-ELISA technique. The result showed that : mean OD values obtained through this measurement was 0.084 for P01, 0.105 for P02, 0.335 for P1 and 0.149 for P2 and the statistical analysis usingDuncan’s multiple range test 5% indicated significant difference (p<0.05) between P0, P1, and P2. The conclusion based on the analysis above, there was a difference antigenicitybetween antigen of Ascaridia galliagainst anti-Ascaridia galli compared antigen of Ascaridia galli against anti-Raillietina tetragonawhich was observed from the OD value average difference of P1 was bigger than P2.

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