AHMAD KURNIAWAN, 061011010 (2014) UJI EFEK IMUNOSTIMULAN EKSTRAK ETANOL DAUN TEKELAN (Chromolaena odorata L.) TERHADAP AKTIVITAS SEL MAKROFAG MENCIT YANG DIINDUKSI BAKTERI Staphylococcus aureus. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This research was conducted to investigate the immunostimulant effect of ethanolic extract of tekelan leaf (Chromolaena odorata L.) for macrophage activity on mice induced by Staphylococcus aureus. Twenty five of two months old male mice BALB/c strain with 25-30 gram average body weight was divided into five group P0; P1; P2; P3; and P4. P0 is positive control was given Stimuno® product P1 is negative control was given CMC Na 0,5%, and P2, P3, P4 was given dosage 250 mg/kg BB, 500 mg/ kg BB, 750 mg/kg BB of ethanolic extract tekelan leaf. Provision of treatment carried out for 10 days after the first conducted animal acclimatization for 7 days and on day 11, mice were injected suspension of Staphylococcus aureus traperitonial were 107 CFU/ml are already synchronized with McFarland solution. Then wait for 1 hour and mice euthanized by neck dislocation and taken peritoneum liquid preparations to stained. Stained preparations in object glass with Giemsa stain and observed under a microscope with 400X magnification by counted the number of active macrophages every 10 field of view. The result showed the ethanolic extract of tekelan leaf increased activity of active macrophage

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