MAHATMA AYU KHALIFA RAMADHANI, 061011263 (2014) PROFIL PROTEIN CACING DEWASA Eurytrema pancreaticum DENGAN TEKNIK SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS (SDS-PAGE). Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this research was to find out protein profile of adult stage Eurytrema pancreaticum by using sodium dodecyl sulphate polyacrilamide gel electrophoresis technique. The result of this research can be used as a basis to find a specific proteins of E. pancreaticum by immunoblotting technique, also Ag diagnostic by serologic test. Preparation of homogenates WWE by means of a worm grind with a mortar and then added 3 ml of PBS, then sonikasi 20x 60/sec and centrifuge 5000 rpm for 15 minutes. Two hundred adult worms were isolated from cattle’s pancreas and were incubated in PBS with temperature 37°C for 12 hours, Excretion-Secretion (ES) liquid of worms were produced in PBS isolated with saturated ammonium sulphate 1,076/10ml. Both of them were identified by using SDS-PAGE method. The protein profile of WWE of E. pancreaticum showed there were 15 bands, as followed: 193.7 kDa, 127.4 kDa, 94.6 kDa, 77.8 kDa, 66 kDa, 60.5 kDa, 52.7 kDa, 47.3 kDa, 42.1 kDa, 38.2 kDa, 31.6 kDa, 26.1 kDa, 20.1 kDa, 13.8 kDa, 11.2 kDa and 6 protein bands profile of ES E. pancreaticum, as followed: 69 kDa, 43.1 kDa, 39.2 kDa. 28.1 kDa, 20.1 kDa and 11.2 kDa.

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