KAMAL MUSTHOFA, 060710044 (2011) UJI MOTILITAS DAN VIABILITAS SPERMATOZOA DOMBA DALAM PENGENCER KUNING TELUR SITRAT YANG MENGANDUNG BERBAGAI DOSIS ION PERAK. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The quality of spermatozoa affected the success of an artificial insemination program. The purpose of the study was aimed at determining the percentage of sheep’s spermatozoa motility with egg yolk citrate diluent stored at a temperature containing various doses of Silver Ion Solution (SIS) and determining the viability or the percentage of sheep’s spermatozoa lives by adding egg yolk citrate diluent stored at a temperature of of 50C containing various doses of Silver Ion Solution (SIS). Sperm collecting was conducted once in four days with seven replications and five treatments. The treatments were firstly, P0 treatment which consisted of egg yolk semen diluent mixed with citrate, penicillin and streptomicin (control); secondly, P1 became (A mixture) which consisted of cement mixed with Egg Yolk Citrate diluent (EYC) and silver ions (SI) (1.5 ppm); thirdly, P2 became (B mixture) which consisted of cement mixed with Egg Yolk Citrate diluent (EYC) and silver ions (SI) (0.75 ppm), next, P3 became (C mixture) which consisted of cement mixed with Egg Yolk Citrate diluent (EYC) and silver ions (SI) (0.375 ppm), and the last, P4 became (D mixture) which consisted of cement mixed with Egg Yolk Citrate diluent (EYC) and ion silver (IS) (0.1875 ppm). The result showed that in P3 and P4 treatments, the decrease of motility was relatively stable compared to P1 (P1 treatment decreased drastically). The percentage of survival at P0, P2, P3, P4 found steady decline compared to P1. Further, dose of silver ions which were competent in this study was 0.375 ppm.

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