LAZIMATUL KHULUQIL HASANAH, 060710232 (2011) PERBANDINGAN RESPONS IMUN HUMORAL PADA AYAM YANG DIVAKSIN IBD AKTIF LV-13UA DAN LV-14UA BERDASARKAN NILAI OPTICAL DENSITY INDIRECT ELISA. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Infectious Bursal Disease (IBD) is a disease of chicken caused by Birnavirus . This disease was immunosuppressive and has a mortality rate up to 100%. This study was aimed to prove the existence of differences in humoral immune response in chicken va ccinated with active IBD vaccine LV -13UA and LV -14UA based on the value of Optical Density with Indirect ELISA method. The study was conducted on 21 chicks that were divided into 3 groups (P0, P1, and P2) . Group P1 consist of 7 chicks was vaccinated with a ctive IBD mild strain vaccine at the age of three weeks given 0,3 ml/ chick then boostered with active IBD vaccine LV -13UA at the age of five weeks. Group P2 consist of 7 chicks was vaccinated with active IBD mild strain vaccine at the age of three weeks g iven 0,3 ml/ chick virus then boostered with active IBD vaccine LV -14UA at the age of five weeks . Group P0 (control) consist of 7 chicks was given 0,3 ml normal saline /chick at the age three and five weeks. Blood sampling for value of Optical Density (OD) I BD antibody observations performed three times in all age groups for three weeks, five weeks, and seven weeks . Measurement of value of Optical Density (OD) IBD antibody was us ing indirect ELISA method. Data analysis used the General Linear Models (GLM) an d ANOVA. The result showed that there are differences in humoral immune response in chicken vaccinated with active IBD vaccine LV - 13UA and LV -14UA based on the value of Optical Density . The value of OD antibody of chicken vaccinated with active IBD vaccine LV -14UA is higher than IBD vaccine LV -13UA

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