Yaska Ikarina, 060313173 (2007) PEMERIKSAAN KADAR PROTEIN SPESIFIK HASIL ELUSI Aeromonas hydrophila YANG DIISOLASI DARI IKAN MAS (Cyprinus carpio Linn)DENGAN METODE BRADFORD PROTEIN ASSAY. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This research was aimed to determine protein level of Aeromonas hydrophila with quantitative measurement Bradford protein assay by knowing highest level of protein. Aeromonas hydrophila were isolated from common carp (Cyprinus carpio Linn) which got from Balai Benih Ikan Punten, Batu, East Java. Identification were done using biochemical test and were cultured on spesific media Triptone Soya Agar (TSA). Protein characterization were analized through some stages : creating whole protein, protein characterizing using SDS-PAGE, rabbit immunization with whole protein, determining immunogenic protein using western blotting, protein purifiying with elution techniques, and Bradford protein assay supported to measure spesific protein level of Aeromonas hydrophila. The result showed that Aeromonas hydrophila protein molecular weight 71,4 kDa; 61,7 kDa; 53,7 kDa; 41,5 kDa; 35,2 kDa its protein level is 0,53 gr%; 0,67 gr%; 0,62 gr%; 0,63 gr%; 0,6 gr%. The highest spesific protein level is reached at protein fractions 61,7 kDa.

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