ZAYYIN DINANA, 061011205 (2014) REAKSI SILANG ANTIGEN Raillietina tetragona DENGAN ANTIBODI TERHADAP Raillietina echinobothrida MENGGUNAKAN METODE ELISA. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This research is conducted to examine the cross-reaction between antigens of Raillitiena tetragona worm and antibodies of Raillietina ecinobothrida using indirect-ELISA technique. This research is divided into two phases. The first phase consist of isolating and identifying adult Raillietina spp. worms, creating homogenate, measuring protein content of the homogenate, and creating polyclonal antibody of mouse (Mus musculus). The second phase consist of indirect-ELISA. The result of indirect-ELISA is measured using ELISA reader with 405 nm wave-length. Optical Density (OD) values obtained through this measurement is 0.105 for (P01) and 0.101 for (P02) control serums, 0.237 for anti-R tetragona serum (P1), and 0.293 for anti-R. ecinobothrida serum (P2). The result of statistical analysis using F (one way ANOVA) 1% test indicates significant difference (P< 0.01). Based on Duncan 1% test advanced analysis, there is a very significant difference (P< 0.01). Based on the analysis above, there is cross-reaction, because OD value of (P2) is higher than (P1). It means Raillietina tetragona antigens can be identified by anti-Raillietina ecinobothrida serum.

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