NUR ZAHROTUL HAYATI, 060313208 (2007) IDENTIFIKASI DAN KARAKTERISASI PROTEIN GROWTH DIFFERENTIATION FACTOR-9 (GDF-9) YANG DIISOLASI DARI OOSIT PADA FOLIKEL DOMINAN OVARIUM SAPI. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This research comprises a series of researches on protein Growth Differentiation Factor-9 (GDF-9) fraction in in vitro maturation process to improve the quality of oocytes as oocyte bank for in vitro embryo production. The research is aimed at identification and characterization of protein Growth Differentiation Factor-9 (GDF-9) isolated from oocytes dominant follicles of bovine ovum. Bovine ovary obtained from a slaughterhouse was aspired in its ovary follicles then extracted by sonication technique. Identification of protein Growth Differentiation Factor-9 (GDF-9) fraction was carried out by means of Sodium Dodecyl Sulphonate Polyacrylamide Gel Electrophoresis (SDS-PAGE). From the results of research, several protein fractions were obtained. Based on the calculation of regression equation resulting from protein marker to determine the molecular weight of Growth Differentiation Factor-9 (GDF-9) protein, 8 protein fractions were determined, they are : 146.46 kDa, 116.12 kDa, 101.02 kDa, 51.06 kDa, 39.94 kDa, 34.75 kDa, 31.67kDa, 15.07kDa. Protein appearing in the protein band the molecular weight of 51.06 kDa was identified as Growth Differentiation Factor-9 (GDF-9) protein fraction playing a role in follicle development.

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