ANDI MAHAKISTA, 060112916 (2007) PENGARUH LAMA SIMPAN SAMPEL DAN PENAMBAHAN SERUM TERHADAP TINGKAT KEBERHASILAN KULTUR PRIMER SEL SOMATIK DARI JARINGAN KULIT TELINGA RUSA BAWEAN (Axis kuhlii) UNTUK KONSERVASI PLASMA NUTFAH. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Primary cell culture potentially is able to the produce somatic cells as a cell lines. As these cells may be able to provide an unlimited cell source to produce embryo by nuclei transfer method. The research that we used Bawean deer auricle epiderm as a sample was to obtain the growth yield level on primary tissue culture by in vitro. The first experiment was to obtain the level of yield primary tissue culture with sample that we preserved into Phosphat Buffer Saline solution during 0, 24, and 48 hours on room temperature. Furthermore, the second experiment was doing in order to differentiate the using of Fetal Bovine Serum 10% and Estrous Goat Serum 10% as an addition in primary tissue culture by TCM 199. The variable of research which we observe last time was include tissue attachment time, the cell growth level, cell viabilities and cell concentrations. A result of first experiment indicate a sample with we preservation term of 0 hour could attach and growth until being confluens, eventhough a sample which we preserved during 24 and 48 hours couldn’t attach and growth very well. A result of second experiment that increased at FBS 10% on culture medium was indicate a tissue attachment in 5th days of culture; 50% level of cell growth could be between 28th – 32nd days of culture and being confluens on 43rd days of culture; average of cell viabilities reached 59,7%; average of cell concentrations reached 1,88 x 105 cells/ml. Eventhough a result of experiment that increased at EGS 10% on culture medium was indicate a tissue attachment in 4th days of culture; 50% level of cell growth could be increased between 25th – 28th days of culture that couldn’t be confluen cause a tissue culture being contaminated between 39th – 43rd days of culture so that we couldn’t know both the cell viabilities and cell concentrations.

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