Novalia Dewi, 060413304 (2008) ANTIGENITAS Brucella abortus STRAIN 19 TERHADAP ANTIBODI POLIKLONAL DENGAN METODE WESTERN BLOT. Skripsi thesis, UNIVERSITAS AIRLANGGA.
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Abstract
The objectives of this research were to detect antigenic strain 19 of Brucella abortus based on specific protein molecular weight characteristic. Polyclonal antibody was produced by injecting subcutaneous the suspention which consist of Brucella abortus S-19 and Complete Freund’s Adjuvant to male rabbit. The booster had been done for six times with ten days interval by injecting suspention tha contain of Brucella abortus S-19 and Incomplete Freund’s Adjuvant Blood sampling was collected from male rabbit in order to get polyclonal antibody anti Brucella abortus S-19, ten days after the last booster. Fractination of protein antigen base on molecul weight was performed by using SDS PAGE technique. Western Blot technique was conducted to find out antigenicity of Brucella abortus S-19 to antibody polyclonal anti Brucella abortus S-19. The results of this reseach showed that there are four bands of protein from Brucella abortus S-19 which were recognized antibody with molecul weight of 64,2 kDa, 23,9 kDa, 21,2 kDa, and 19,0 kDa.
| Item Type: | Thesis (Skripsi) | |||||||||
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| Additional Information: | KKC KK KH . 133/08 Dew a | |||||||||
| Uncontrolled Keywords: | BRUCELLA ABORTUS S-19, PROTEIN, WESTERN BLO | |||||||||
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology > QR75-99.5 Bacteria S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine |
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| Divisions: | 06. Fakultas Kedokteran Hewan | |||||||||
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| Depositing User: | Nn Dhani Karolyn Putri | |||||||||
| Date Deposited: | 24 Aug 2010 12:00 | |||||||||
| Last Modified: | 01 Oct 2016 07:24 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/21769 | |||||||||
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