ADY KURNIANTO, 061224253004 (2016) PROFIL SITOKIN (TNF-α dan IFN-γ) DAN PERUBAHAN HISTOPATOLOGI HEPAR PADA TIKUS PUTIH (Rattus novergicus) YANG DI INFEKSI Trypanosoma evansi ISOLAT SUMBAWA (P029) PENELITIAN EKSPERIMENTAL LABORATORIS. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Cytokines TNF-α and IFN-γ as the immune system plays an important role in the immune response against Trypanosoma evansi. Organ liver as metabolime center is one of the organs that were damaged by the metabolism of Trypanosoma evansi antigen in the body. Usage samples Sumbawa Trypanosoma evansi isolates have the nature of the pathogen and constant profile in infections in animals. Subcutan infection pathways to a period prepaten slower than the intraperitoneal pathway in animal infections. The purpose of this study was to analyze the value of Optical Density (OD) cytokines TNF-α and IFN-γ and analyze the degree of liver damage in rats (Rattus norvegicus) were infected by Trypanosoma evansi isolates Sumbawa (P029). The subjects of this study using rats (Rattus norvegicus) from Winstar strain, male, 200-250 gram weight, 30 rats were divided into 5 groups. Grouping consists of the control (physiological NaCl) and 5 groups were infected with T. evansi. Dose rats treated control group (physiological NaCl) 0.3 ml/sc and white rat treatment groups of 0.3 ml/sc (containing T. evansi 106 from mice Bogor) subcutan. Blood sampling and hepatic organ in the rat control group P0 (7 days post injection of physiological saline), P1 (1 day post-infective T. evansi), P2 (3 days post-infective T. evansi), P3 (5 days post-infective T. evansi) and P4 (7 days post-infective T. evansi). The results show the value of Optical Density (OD) of TNF-α and IFN-γ decreased significantly were not significantly different (p>0,05) and the correlation between TNF-α and IFN-γ there is no significant relationship (p>0,05) the white ratsinfected by Trypanosoma evansi isolates Sumbawa (P029). Effect of Trypanosoma evansi infection isolates Sumbawa (P029) subcutan white rats can cause liver damage in the form of lesions degeneration, necrosis and inflammation portal. Need examination of cytokines or other hormones and the use of immunohistochemical methods (IHC) in the case of Trypanosomiasis. Factors to consider in the use of experimental animals include animal health, room or enclosure density, and the status of good nutrition so expect to get good results and appropriate.

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