RIO DARLIS AHYARI, 061314453001 (2016) EFEKTIFITAS ANTIBODI ANTI-HA ASAL KUNING TELUR (IgY) SEBAGAI AGEN IMUNOTERAPI PADA AYAM YANG DIINFEKSI VIRUS AVIAN INFLUENZA A/H5NI CLADE 2.3.2 PENELITIAN EKSPERIMENTAL LABORATORIS. Thesis thesis, UNIVERSITAS AIRLANGGA.
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Abstract
The aims of this research are: (1) to analyze the activity of Avian influenza virus subtype H5N1 clade 2.1 anti-HA antibody against the infection of Avian influenza virus subtype H5N1 clade 2.3.2 by observation of antigen in cell tropism through IHC preparations. (2) to analyze the blocking mechanism of anti-HA antibody towards AI virus in cell tropism through SA alfa 2.3 gal receptor. (3) to analyze the potential of anti-HA by the dose of 400μg, 200μg, 100μg and 0μg, as an immunotherapeutic product based on its protection value given by infection dose of AI virus subtype H5N1 in chicken, by survival rate observation. This research is divided into three stages. First stage is the formulation of immunotherapy agent anti-HA antibody in various concentrations and preparation of virus for challenge test. Second stage is the challenge test and artificial infection in chickens by administering the immunotherapeutic agent at different times. Third stage is the observation of immunohistochemical preparations to detect the presence of Avian Influenza virus antigen and anti-HA antibody binding in cell tropism. The results showed that: (1) Egg yolk derived anti-HA clade 2.1 antibody could bind HA protein antigen and could protect chickens from clade 2.3.2 Avian influenza A / H5N1 virus infection. (2) Protection mechanism from Avian influenza A / H5N1 virus infection occurs via the blocking of SA alfa2.3 gal receptor. (3) The anti-HA antibody exhibits the highest protective activity when administered on the day before Avian influenza virus infection by the dosage of 400μg and 200μg with protection value reaching up to 80 % -100%.
| Item Type: | Thesis (Thesis) | |||||||||
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| Additional Information: | KKC KK TVI.03/16 Ahy e | |||||||||
| Uncontrolled Keywords: | anti-HA antibody, immunoglobulin Y, Avian influenza A/H5N1 clade 2.3.2 | |||||||||
| Subjects: | S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF780.2-780.7 Veterinary microbiology, bacteriology, virology, mycology | |||||||||
| Divisions: | 06. Fakultas Kedokteran Hewan | |||||||||
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| Depositing User: | Guruh Haris Raputra, S.Sos., M.M. '- | |||||||||
| Date Deposited: | 20 Apr 2016 07:41 | |||||||||
| Last Modified: | 20 Apr 2016 07:41 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/29584 | |||||||||
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