BUDI UTOMO, 090710352 D (2011) SUPLEMENTASI AKROSIN PADA SEMEN KAMBING PERANAKAN ETAWA (PE) PASCA THAWING TERHADAP PENINGKATAN KUALITAS DAN POTENSI SPERMATOZOA (Penelitian Eksperimental Laboratoris). Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The development of goats population in Indonesia has not reached a satisfactory condition, in East Java in the 2007 population of goats decreased for about 3,24%. (Anonymous, 2007).The obstacle which is faced in the goats breeding field is involving reproduction, the problem includes failure sperm cell to penetrate zona pellucida on egg cell due to the of less potency enzyme acrosin and this is the first factor which hampers goats reproduction. This research consisted of two experiment. The first experiment was biological test for determining the potency of spermatozoa after thawing supplemented with acrosin at the dosages of 0; 3.0; 4.5 and 6.0 for 30 and 60 minutes respectively. This biological test including that for motility, viability, abnormality, capacity, non capacity and acrosome reaction. The second experiment was to test the fertility of that spermatozoa for their capability to fertilize ova in vitro. The results were, in the first experiment supplement of acrosin 4.5 µg increased motility goat PE’s spermatozoa. Supplement of acrosin 3.0 and 4.5 µg increased viability, capacity and spermatozoa goat acrosom reaction. In the second experiment the percentage of cleavage of oocytes were 40; 60; 60; and 0 for spermatozoa supplemented with 0; 3.0; 4.5 and 6.0 of acrosin respectively. The conclusion were supplement acrosin with dosage 4.5 µg increased quality of sperm and acrosin supplement 3.0 µg for 30 minutes increased potency of sperm.

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