ROEDI IRAWAN, 090710143 D (2011) EFEK PEMBERIAN GLUTAMIN TERHADAP AKTIVITAS SUCRASE, MALTASE, LACTASE DAN EKSPRESI SPECTRIN, CLATHRIN DALAM PERBAIKAN MIKROVILI USUS PADA TIKUS MALNUTRISI. Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Back groud: Glutamine is the most abundant amino acid in the blood and plays a key role of intestinal response to local and systemic injuries such as diarrhea and malnutrition. Malnutrition occurs interference in intestinal enzyme activity. Obyective:The purpose of this study is to explain the effect of oral glutamine on sucrase, maltase, lactase enzyme activity and spectrin, clathrin expression also the correlation with ERK-1 and STAT-3 signaling pathways in repairing microvilli intestine in rat malnutrition. Method: The method employed in this study was experimental research, with control samples, measurable and reliable, with the design of the study was post test only group design. showed that twenty growing male rats (aged 12 week) were divided into 4 groups: control, malnutrition, control+glutamine and malnutrition+glutamine. For the first 15 days experiment, animals in the test groups received a karak diet (low calorie diet of dried rice) for the next 15 days. Control and malnutrition groups received either glutamine supplement with the doses 500 mg/kg body weight/day. On the 30th days, the animals were weighed and sacrificed of the intestine was taken and prepared for enzymatic and expression examination. Results: Sucrase activity between malnutrition with malnutrition+glutamine groups there indicated no significant differences in duodenum, but significance in jejunum (p = 0.04) and ileum (p = 0.04). Maltase activity when compared malnutrition with malnutrition+glutamine groups there were no significant differences in duodenum and jejunum, but significantly in the ileum (p = 0.00). Lactase activity when compared between malnutrition with malnutrition+glutamine groups there were significant differences in duodenum (p = 0.03), jejunum (p = 0.04) and ileum (p = 0.04). The expression of spectrin and clathrin were evidence enlarge of enterocyte in the normal group after being given glutamine compared to malnutrition group after being fed glutamine, spectrin and clathrin expression similar appearance on jejunum and ileum. Spectrin and clathrin molecular weight protein in the duodenum, jejunum and ileum were the same, with a molecular mass of 130-250 KDa. Relationship between glutamine and meaningful pathway signaling proteins ERK-1 in the duodenum (p = 0.00), jejunum (p = 0.00) and ileum (p = 0.03) and also significant association between glutamine and STAT-3 in the duodenum (p = 0.00), jejunum (p = 0.00) and ileum (p = 0.00). Conclusion : The research proved that there was an increase activity of sucrase, maltase, lactase, also spectrin and clathrin which have expression differences of each group. There are links between ERK-1 and STAT-3 signaling pathway in the repairing microvilli of rat intestinal epithelial mucosa damaged by malnutrition and improved after administration of oral glutamine

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