AGUS SULISTYONO, 090810124 D (2012) PENINGKATAN L-TYPE CALCIUM CHANNEL, KALSIUM INTRASELULER, PAR-1 dan HSP-27 pada UTERUS KELINCI NEW ZEALAND YANG DILAKUKAN PENJAHITAN MODIFIKASI SURABAYA (KAJIAN BIOMOLEKULER PADA PENANGANAN PERDARAHAN PASCA PERSALINAN KARENA ATONIA UTERI). Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Objective : Uterine atony as the common cause of PPH which causing high maternal mortality can indeed be largely rescued by compression with conservative surgical procedure by performing Surabaya suture compression modification (SSCM). To unveil the biomolecular phenomena after Surabaya compression suture leading myometrium contraction, the purpose of this study is to analyze the increased expression of L-type calcium channel, PAR-1 and HSP-27 and density of intracellular calcium on animal model of New Zealand rabbit myometrium after procedure performance Material and methods: True experimental single blinded study was done in Airlangga University Veterinary Faculty and Brawijaya University Physiology Department from March – August 2011. Twenty two samples were obtained over this study which applied on aterm pregnant rabbit uterus as animal model. Animal model consisted of rabbit uterus in which practiced on by SSCM in 11 samples after terminating pregnancy and removing pup and placenta as well, and the remainder of another 11 samples without SSCM as the control. The assessment of L-type calcium channel, PAR-1 and HSP-27 expression and density of intracellular calcium on myometrium sample was done respectively by immunohistochemistry and fluoro-2 double staining method at ½,1,2, and 24 hours intervals Result: At all 4 time intervals the increased expression of L-type calcium channel was observed significantly in SSCM compared to control (p=0.001; p< 0.0001; p=0.011 and p<0.0001), also for intracellular calcium density (p=0.022; p=0.001;p=0.005 and p=0.002). The study showed PAR-1 and HSP-27 expression increasing sharply that statistically analysis indicated both all of p similarly < 0.0001 against control. Conclusion: SSCM suggested strongly increasing some component (all variable) that absolutely required to myometrium contraction

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