FERRY YUSRIZAL, 090515739 D (2012) ANALISIS POLIMORFISME A52G DAN C32T GEN KIR3DL2 PADA PREEKLAMPSIA DAN KEHAMILAN NORMAL DI RSUP dr. MOHAMMAD HOESIN PALEMBANG. Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background : Preeclampsia is usually called as a disease of theories where the real etiology still unclear. Preeclampsia is a medical complication of pregnancy which is characterized by hypertension and proteinuria after 20 week pregnancy. Polymorphism of the KIR genes might be associated with the genetic predisposition of preeclampsia. It is found that the KIR3DL2 gene expression was obviously decreased in preeclampsia and assumed that decreased KIR3DL2 gene expression weakened the protection of trophoblasts from being attacked by maternal immunological cell. It causes insufficient utero-placental blood flow and the defective placentation. Objectives : To analyze the polymorphisms of A52G exon 3 and C32T exon 9 Killer cell immunoglobuline-like receptor 3 domains, long cytoplasmic tail, 2 (KIR3DL2) gene in preeclampsia and normal pregnancy in Mohammad Hoesin General Hospital Palembang. Methods : The research used cross sectional study design. There are 63 pregnant women were enrolled. The samples were divided into two groups. The 33 subjecs with preeclampsia and 30 subjects normal pregnancy where their A52G in exon 3 and C32T in exon 9 polymorphism of the KIR3DL2 gene were determined by polymerase chain reaction (PCR) from venous blood samples and the positive result of PCR would be continued to sequencing and multiple sequence alignment. Results : No significant difference statistically among the characteristics of the subjects preeclampsia and normal pregnancy, such as age, gestation, parity and body mass index (p >0,05). The polymorphism of A52G exon 3 KIR3DL2 genes in preeclampsia group are more frequent than the normal pregnancy group where genotype frequency GG (87,9%) in PE is higher than NP (76,6%) and also the alleles G proportionately higher in PE (92,4%) than NP (85,0%), but there is no significant difference statistically between two group PE and NP for genotypes and alleles frequencies. No polymorphism was found in C32T exon 9 KIR3DL2 genes in all subjects of two groups PE and NP. But, there were discovered other polymorphisms after the nucleotide 32 exon 9 in all subjecs of PE and NP. The polymorphisms are in nucleotide sequences 62, 77, 79,1 06/107, 119, 125/126 and 173/174. Conclution : The A52G exon 3 KIR3DL2 genes polymorphism and the C32T exon 9 KIR3DL2 genes were not genetic predisposition for the development of preeclampsia. The mutation of these two sites is not associated with the risk of preeclampsia in preeclamptic patients in Mohammad Hoesin General Hospital Palembang. It looked like that polymorphisms in certain area have the genetic variations in different geographic area or ethnic

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