ERMA SAFITRI
(2014)
PREKONDISI HIPOKSIA UNTUK MENCAPAI LONG TERM MAINTENANCE (LTM) DARI QUIESCENCE BONE MARROW MESENCHYMAL STEM CELLs (BMSCs).
Disertasi thesis, UNIVERSITAS AIRLANGGA.
Abstract
In vitro conventional culture from BMSCs with high oxygen tension (OY-2OOft,) reported by other researchers caused senescence cells formation, apoptotic, and gene mutation. It caused the reduction of stem cells viability before 7th transplantation. The stem cels experienced to death between 93-99% in 1st days after transplantation. Low O2 tension (hypoxia) is needed as condusive microenvironment supporting for in vitro culture in order to maintain viable and adaptive condition at the time of transplantation. This condition is called long term maitenance (LTM). LTM occur if the stem cells reside at silent condition (00), is not experienced cycling state (GIIS/G2IM), but still in proliferation and undifferentiated. This condition in vivo is called as quiescence cells. This research was done in five phases: First pbase was isolation and culture of MSCs from bone marrow of healthy male rabbit (New Zealand strain); Second pbase was BMSCs characterisation by identification of p63 protein as quiescence cells marker, identification ofCD44, CD90, CD73, CDIOS and CD45 before hypoxia precondition;Tbird pbase was hypoxia precondition treatment on the stem cells culture of several 02 concentration (21, 1 and 3%) and several cultivation time (1, 2, 4, 8 days); Fourth pbase analysed four main keys of quiescence LTM such as slow proliferation, optimal concentration of ROS intracellular, plurlpotency and undifferentiated cells; Fifth pbase was Quiescence Cells BMSCs characterisation by identification of p63 protein expresion after hypoxia precondition The overall results were: 1. Slow proliferation based on least like CFU-Fs at The new discovery from this research was L TM quiescence BMSCs can be made by hypoxia precondition based on four of main keys such slow proliferation, optimal concentration of ROS intracellular, expresion of pluripotency factor OCT4 and SOX2, and undifferentiated from BMSCs by genotype of CD44 or phenotype ofCD44, CD90, CD73, CDlO5 and CD45. In conclusion the results of the research was LTM quiescence BMSCs can be obtained by given of hypoxia precondition with optimal consentration of 02 1% for 2 days. UNTUK FULLTEXTNYA LIHAT LANGSUNG PADA HARDCOPY DISERTASI
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