Uswatun Hasanah (2015) Efek Ekstrak Etanol Daun Sirsak (Annona muricata L.) Terhadap Hambatan Pertumbuhan Dan Perubahan Struktur Dinding Sel Bakteri Acinetobacter baumannii. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This research is to prove the antibacterial effect of soursop leaf extract on A.baumannii bacteria in vitro, to find out the correlation of giving phased concentration of soursop leaf extract on the inhibition of A.baumannii bacterial growth in vitro, and to find out the change of the cell structure of A.baumannii bacteria in giving the extract of soursop leaf. Extraction of soursop leaf with maceration method and ethanol dissolvent 96%. The data analysis of antibacterial effect of soursop leaf on growth inhibition of A.baumannii was ANOVA test with standard error of 5%, the correlation of giving phased concentration of soursop leaf extract on the growth inhibition of A.baumannii used Pearson correlation, and the results of SEM analysis was explained descriptively. The test result of antibacterial showed that the extract of soursop leaf had antibacterial activity on A.baumannii bacteria with MIC at concentration 2x104 μg/ml. There was correlation between giving phased concentration of soursop leaf extract and growth inhibition of A.baumannii bacteria where the higher the concentration of the extract, the fewer the number of the bacteria colony. The morphology of A.baumannii bacteria in concentration 0 μg/ml showed normal A.baumannii bacterial cell with cocobasil shape. In concentration 0,5x104 μg/ml, the bacterial cell morphology in cocobasil shape with smaller size than that of control concentration. In concentration 1x104 μg/ml, some of the bacterial cells were in lysis and the size became smaller again. In concentration 1,5x104 μg/ml, most of the bacterial cells were smaller than other concentrations. Meanwhile in concentration 2x104 μg/ml, it was suspected that the antibacterial activity of soursop leaf extract could lysis the bacterial cell so that there was no bacterial growth in SEM analysis.

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