IRA ARUNDINA, 099913410M (2002) PENGARUH CA TECHIN TERHADAP TITER IMUNOGLOBULIN G, JUMLAH DAN DAYA INGESTI PMN PADA MENCIT YANG DIPAPAR Actinobacillus actinomycetemcomitans Penelitian Eksperimental Laboratoris. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Catechin obtained from green tea extract is a natural anti-inflammatory and immunomodulator that can be used as alternatives to relieve immune system disorder. The objective of this study was to identify the influence of catechin on immunoglobulin G titer and polymorphonuclear (PMN) phagocytosis activity in mice exposed to Actinobacillus actinomycetemcomitans (A.a.). This study was a laboratory experimental study with complete random design. Subjects were male Swiss Webster (ba/b-c) mice of 2-3 month-old with bodyweight of 25 - 30 gr, divided into 4 groups. Group 1= 7 mice were given with catechin of 0.1 gr/kgbw/d orally once each day. Group 2= 7 mice were given with catechin of 0.1 gr/kgbw/d orally once each day and at day 8 the mice were exposed to A.a. intraperitoneally. Group 3= 7 mice were given with Carboxyl Methyl Cellulose (CMC) as solvent for catechin once each day orally and at day 8 the mice were exposed to A.a intraperitoneally. Group 4= 7 mice were given with CMC as solvent for catechin once each day orally. Treatment was done for 25 days, and at day 11, 18, and 25 IgG titer was examined using ELISA test. Data obtained were analyzed by the use of Anava test. If significant difference was found, the analysis had been continued by using Duncan's test. At day 11, PMN number and ingestibility were examined by swab preparation, giemsa staining, and observation by means of microscope with 1000 times magnification. Data obtained were analyzed using t test. Results of IgG titer examination showed increased IgG titer at day 18 compared to that at day 11, and decreased IgG titer was found at day 25 compared to that of day 18. However, the decreased value at day 25 was still higher than that at day 11. Those results were observed in all treatment groups. Results oft test showed that catechin could reduce PMN number and ingestibility. In conclusion, catechin may increase IgG titer and reduce PMN number and ingestibility in mice exposed to A.a.

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