SUPARNIASRI (2007) PENGARUH PEMBERIAN EKSTRAK BUNGA ROSELLA (Hisbiscus sabdarifa. L) TERHADAP KUANTITAS DAN KUALITAS SEL SPERMATOGENIK PADA MENCIT (Mus musculus) YANG DIBERI 2-METHOXYETHANOL. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The goal of this research was known the influence Rosella flower's (Hisbiscus sabdarifa L.) extract to quality and quantity of spermatogenic cells of mice (Mus musculus) that be given 2-Methoxyethanol (2-ME). The study used 35 male adult mice (sexually mature). Those divided into 5 groups, each group consist of 7 mice. One group (negative control group) be given 2-ME solution, and three treatments group be given Rosella flower's (Hisbiscus sabdarifa L.) extract in dosages 14 mg/kg BW/day, 28 mg/kg BW/day, and 56 mg/kg BW/day. The solution was given orally for 5 days for control group and 13 days for treatment groups. The treatment group had been given 2-ME solution for five days before got Rosella flower's (Hisbiscus sabdarifa L.) extract. After the treatment, the spermatozoa was taken from epididimis was observed for mortality, morphology, and the amount of spermatozoa and than was made testis fixation by Hematoxillin Eosin (HE) fixation to account the amount of spermatogonium, primary spermatosit, and spermatid.The result were (1) there was significant different among 2-ME group and all of Rosella flower's (Hisbiscus sabdarifa L.) extract group for spermatozoa motility; (2) there was significant different among 2-ME group and all of Rosella flower's (Hisbiscus sabdarifa L.) extract group for spermatozoa morphology; (3) there was significant different among 2-ME group and all of Rosella flower's (Hisbiscus sabdarifa L.) extract in dosages: 28 mg/kg BW/day, 56 mg/kg BW/day for amount of spermatozoa; (4) there was significant different among 2-ME group and all of Rosella flower's (Hisbiscus sabdarifa L.) extract groups for amount of spermatogonium; (5) there was significant different among 2-ME group and group of Rosella flower-s (Hisbiscus sabdarifa L.) extract in dosages: 14 mg/kg BW/day, 28 mg/kg BW/day, 56 mg/kg BW/day for amount of primary spermatosit; and (6) there was significant different among 2-ME group and all of Rosella flower's (Hisbiscus sabdarifa L.) extract groups for amount of spermatid.The conclusion was Rosella flower's (Hisbiscus sabdarifa L.) extract could increase the motility of spermatozoa, normal morphology, number of primary spermatosit and amount of spermatid in mice that be given 2-ME.

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