PRATIWI SOESILAWATI, 090710155 D (2011) ANALISIS VARIAN HLA-DRB1 PADA JALUR IMUNOGENETIK SEKRESI sIgA SALIVA SEBAGAI RISIKO KARIES GIGI (PENELITIAN PADA POPULASI JAWA DI SURABAYA ). Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

HLA-DRB1 allele was derived from MHC class II molecules and play an important role in the control of antigen peptides presentation to TCR that encoded sIgA secretion which contribute to prevent S. mutans colonization. The purpose of this study were to determine the role of HLA-DRB1 polymorphisms that affect the CD4 count and TGF-1 expression on immunogenetic pathway of sIgA secretion in saliva and raises the risk of dental caries. Methods of this research were DNA analysis on case and control sample groups with low and high levels of sIgA by ELISA test. HLA-DRB1 polymorphisms were studied through DNA isolation for PCR-RFLP proceeded with sequencing. Immunogenetic pathway of sIgA secretion was studied through the relationship between HLA-DRB1 variant to CD4 count and TGF-1 expression. Results proved that nomenclature of HLA-DRB1 variant DRB * 1209 (2), DRB * 1209 (3) and DRB * 1209 (4) were associated with low sIgA levels and high caries risk. Whereas DRB * 1209 and DRB * 1209(1) were associated with high sIgA levels and low caries risk. Path analysis showed that CD4 affects sIgA via TGF-1, because TGF-1 directly influence the sIgA and CD4 effect on TGF-1. The study has concluded that mutation in HLA-DRB1 alleles cause changes in sIgA levels and therefore contributes to the risk of dental caries. HLA-DRB1 polymorphisms contributes to the change in CD4 cell count and changes in expression of TGF-1 on the immunogenetic path of sIgA secretion.

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