Deby Kusumaningrum, NIM010942004 (2011) Deteksi Mutasi Regio gen gyrA Mycobacterium tuberculosis Resisten Terhadap Fluoroquinolon. Thesis thesis, UNIVERSITAS AIRLANGGA.
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Abstract
Background: Fluoroquinolones are an important class of antibiotics used in the management of bacterial diseases and drug resitance tuberculosis (TB).This study aimed to determined the mechanism of fluoroquinolon resitance in Mycobacterium tuberculosis by sequencing of the quinolone resistance determining region (QRDR) of gyrA, one of the genes encoding DNA gyrase. Mutations affecting amino acids at positions74,90,91 and 94 have been most commonly associated with resistance . Methods: 1 Drug susceptibility testing of Mycobacterium tuberculosis was performed by the method of proportion, including susceptibility to ofloxacin. Isolates were from sputum specimens of patients treated at the Pulmonary lung Hospital (BP-4) Surabaya East Java (2) DNA was extracted by boiling method. (3) Nucleic acid was amplified by PCR, targeting the region of gyrA using primers ": primer gyrA-F ( 5'CAG CTA CAT CGA CTA TGC GA 3") dan gyrA-R( GGG CTT CGG TGT ACC TCA T) and sequenced with ABI 377 sequencer. (4) Sequences were analysed to identify mutations associated with fluoroquinolone resistance. Result: 9 of 20 isolates tested were ofloxacine resistant Mycobacterium tuberculosis . PCR for gyrA produced amplicons of 320 bp from five these isolate. No mutations were identified in gyrA ( specifically at sites corresponding with codons 90,91,94) but all 5 isolates had the mutations S95T. Conclusion: these findings suggest that the gyrA mutation, S95T is polymorphism in Fluoroquinolone resistant Mycobacterium tuberculosis, in Surabaya. However , further investigation is required to identify the mechanism of Fluoroquinolone resistance in Mycobacterium tuberculosis.
| Item Type: | Thesis (Thesis) | |||||||||
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| Additional Information: | KKA KK TKD.06/13 Kus d; KKB KK-2 TKD.06/13 Kus d; KKC KK TKD.06/13 Kus d | |||||||||
| Uncontrolled Keywords: | Fluoroquinolone Resistance, Mycobacterium tuberculosis, gyrA , gene mutation | |||||||||
| Subjects: | R Medicine > RC Internal medicine > RC306-320.5 Tuberculosis | |||||||||
| Divisions: | 01. Fakultas Kedokteran | |||||||||
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| Depositing User: | Nn Husnul Khotimah | |||||||||
| Date Deposited: | 2016 | |||||||||
| Last Modified: | 11 Oct 2016 05:57 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/37041 | |||||||||
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