YUANI SETIAWATI, 090810198 (2011) EFEK ANTIBAKTERI Streptomyces sp-MWS1, Streptomyces sp-MWS3, DAN Streptomyces sp-MWS6 TERHADAP Klebsiella pneumoniae NON-PENGHASIL Extended-Spectrum -Lactamase (ESBL) DAN Klebsiella pneumoniae PENGHASIL Extended-Spectrum -Lactamase (ESBL) PENELITIAN EKSPE. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Klebsiella pneumoniae is one of the causative agent nosocomial infection. The main concern on this bacterium is directed on the extended-spectrum -lactamase (ESBL)-producing Klebsiella pneumoniae. The therapy of ESBL-producing Klebsiella pneumoniae infections is very limited. In mangrove East Coast of Surabaya, it had been found new local isolates Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6. These isolates have potential to produce antibiotics (Retnowati, 2008). This study was aimed to prove these isolates may inhibit the growth of non ESBL-producing Klebsiella pneumoniae and ESBL-producing Klebsiella pneumoniae, and that are differences between the antibacterial activity of these isolates. The test of antibacterial activity of Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 using the modification Agar print method against clinical isolates of non ESBL-producing Klebsiella pneumoniae and ESBL-producing Klebsiella pneumoniae. The diameter of inhibition zone (mm) formed shows activity of these isolates. The profiles of antibacterial activity of Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 were different in terms of periode required to reach peak activity, duration of activity and inhibition zone diameter produced during 10 days of observation. Streptomyces sp-MWS3 reached the peak activity most rapidly on day 3 with the largest inhibition zone diameter of 12 mm in non ESBL-producing Klebsiella pneumoniae and 9 mm in ESBL-producing Klebsiella pneumoniae. These isolates produced larger inhibition zone diameter in the non ESBL-producing Klebsiella pneumoniae. There were significant differences in inhibition zone diameter between Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 against non ESBL-producing Klebsiella pneumoniae and ESBL-producing Klebsiella pneumoniae.

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