MIRWA ADIPRAHARA ANGGARANI, 080942003 (2011) IDENTIFIKASI PROTEIN ANTIGENIK SPESIFIK BIOFILM SEBAGAI KANDIDAT BIOMARKER KANDIDIASIS. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Candida albicans is a human commensal and opportunistic pathogen that participates in biofilm formation on host surface and also in medical devices. Protein profiles of fungal biofilm have not investigated in details, although such profile are believed to play critical roles in fungal biofilm formation. We used Sodium dodecyl sulfate – polyacrilamide gel electrophoresis (SDS-PAGE) joined with Western blotting to analize biofilm specific antigenic protein of C. albicans by investigating the reactivities of antibodies in sera from mice to antigenic protein of biofilm formed on cellulose nitrate membrane compared to planktonic form. The result, we analize the antigenic protein profile of C. albicans biofilm includes proteins with molecular weight 76.4 kDa, 70.6 kDa, 67.1 kDa, and 52.1 kDa. Also, antigenic protein profile of C. albicans planktonic includes proteins with molecular weight 76.4 kDa, 70.6 kDa, 64.8 kDa, 52.1 kDa, 47.4 kDa and 40.3 kDa. Based on comparation of antigenic protein profile of biofilm and planktonic, we analize the biofilm specific antigenic protein, that is protein with molecular weight 67.1 kDa. This biofilm specific antigenic protein of C. albicans, is a candidate to biomarker of candidiasis.

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