Merry Safinah, 080942002 (2011) DESAIN DAN BIOASSAY INHIBITOR PROTEIN Bgl2 PENYUSUN BIOFILM PADA Candida albicans. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Candida albicans is one of the most commonly isolated fungal pathogens in human body and also most commonly associated with biofilm formation. Biofilm protects microorganism from immune system of the host cells and antimicrobials mmunereponses.It is reported that C. albicans biofilms are highly resistance to eleven antifungal compounds such as fluconazole, ketokonazole, amphotericin B, clotrimazole, miconazole, zaragozic acid B, terbinafine, cerulenin, chlorhexidine, and nystatin.Approximately 80 to 90% of the cell wall of C. albicans consist of carbohydrate mostly containing polymers of glucose _-1,3 and _-1,6 linkages (_-glucans), 50 – 60%, mannoprotein 30 – 40%, and chitin 0.6 – 9%. Instead of being cell wall components, Bgl2 composed ectracellular biofilm matrix. Using modeling simulation the research blocking the interaction of Bgl – Bgl to formed biofilm. Based on method 2D-Structure matching, activity relationships represent an attempt to correlate structural or property descriptors of compounds with activities. Kanamicyn are the structure which similarity with _-D-glucose. Second method is Virtual Screening using the docking program such as AutoDock Vina and AutoDock4 and we obtained the docked energy for kanamicyn are -7,71 kcal/mol, and the inhibition constant are 2,23 μM. Kanamicyn has identified the binding interaction at Glu192 and Glu292 which known as the catalytic residues of Bgl2. The effect of 5μM kanamicyn inhibit 61,69% protoplast regeneration and the electro scanning microscopy showed that C. albicans growth was predominantly composed of fragile blastospore.

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