RISTA AFRIDA, 080942013 (2011) POTENSI SENYAWA FENOLIK DARI BUAH KERSEN (Muntingia calabura) SEBAGAI ANTI OKSIDAN ALAM. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Kersen (Muntingia calabura) is one of the Indonesian fruits that has a few economic value but it has potency as antioxidant because of its phenolic compounds. Kersen fruits were extracted in methanol and subjected to solvent-solvent partitioning to yield chloroform and methanol fraction. Total phenolic contents of the methanol extract, chloroform fraction and methanol fraction were estimated by Folin Ciocalteau Reagent (FCR) method. Total phenolic contents in methanol extract, methanol fraction, and chloroform fraction are 43 %, 15,6 % and 5,78 % respectively. Isolation of phenolic compounds were done using gravitation column chromatography method and resulted two compounds ( A and B compounds). The structure of phenolic compounds were identified by spectroscopy method. The antioxidant activity of the methanol extract and two phenolic compounds were tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric thiocyanate (FTC). The extract and two phenolic compounds have DPPH radical scavenging activity and exhibited antioxidant activity using FTC test.

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