Rini Purbowati, 090810599 (2010) EFEKTIVITAS VARIASI JENIS KONSORSIUM MIKROBA DALAM BIODEGRADASI SENYAWA HIDROKARBON AROMATIK PADA TANAH TERCEMAR SOLAR. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Aromatic hydrocarbon compounds was one of the soil pollutant because of its mutagenic, carcinogenic and genotoxic nature. The main source of aromatic hydrocarbon pollution came from petroleum refinary and derived products include diesel fuel. The effectiveness of microbial consortium in soil bioremediation and its derived products was strongly influenced by the ability of each microbe. This study was aimed to find out the microbial consortium isolate able to grow in aromatic hydrocarbon compounds, its potential degradation and the effectiveness of microbial consortium variation (F1, F2, and F3) in biodegradation of aromatic hydrocarbons compound in diesel fuel contaminated soil. Screening of the ability to grow and potential degradation of the microbial consortium isolate were conducted in liquid culture containing monoaromatic hydrocarbons (benzene, toluene, xylene) and polyaromatic (phenantrene and napthalene) at 100 ppm. The microbial growth was observed at 3th and 7th day and the percentage of degradation at 7th. The effectiveness of microbial consortium variation (F1, F2 and F3) in the biodegradation of aromatic hydrocarbons compound in diesel fuel contaminated soil was measured by increasing the Total Heterotrophic Bacteria and Total Heterotrophic Yeast (CFU/g-soil), and the percentage of Total Petroleum Hydrocarbons, total non- aromatic and aromatic compound using GC-FID at 0, 2, and 4 weeks. Total Heterotrophic Bacteria dan Total Heterotrophic Yeast (CFU/g-soil) were analyzed statistically using two-way ANOVA and Duncan test (p = 0.05). Percentage of the Total Petroleum Hydrocarbons, total non-aromatic and aromatic compound were analyzed descriptively. Screening and degradation test showed Pseudomonas stutzeri, Pseudomonas aeruginosa and Actinobacillus sp. were potential to growth and degraded monoaromatic compounds (benzene, toluene and xylene). Pseudomonas stutzeri and Pseudomonas aeruginosa were also potential on sublimation and degradation test on poliaromatic compounds (phenantrene and napthalene). The combination of microbial consortium variation (F1, F2 and F3) and incubation time (0, 2 and 4 weeks) indicated that there are increasing of Total Heterotrophic Bacteria and Total Heterotrophic Yeast (CFU/g-soil) and percentage of the Total Petroleum Hydrocarbons, total non-aromatic and aromatic compound. The best combination was obtained at 2F3 treatment (consortium F3 at second week) with an average of log Total Heterotrophic Bacteria and Total Heterotrophic Yeast (CFU/g-soil) amounted to 19.88, 19.40, and 18.63, and percentage degradation of the Total Petroleum Hydrocarbons, total non-aromatic and aromatic compounds amounted to 46.13%, 45.79%, and 100% respectively.

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