Meilani (2014) PREVALENSI INFEKSI OCCULT HEPATITIS B DAN ANALISIS MOLEKULER PADA REGIO SURFACE VIRUS HEPATITIS B DARI IBU HAMIL DI PUSKESMAS PERAK, SURABAYA. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Perinatal transmission mode is predominant of hepatitis B virus transmission in highly endemic countries of HBV infection. Children born to HBV infected mothers have a high risk of chronicity up to 90% through perinatal transmission. The HBsAg serology screening was recommended to all pregnant women to prevent perinatal HBV infection, but recently HBV DNA could be detected in the serum of patients with negative for HBsAg (OBI). This study was aimed to determine the prevalence of OBI and the virologic characteristics of HBV among pregnant women from Surabaya. Sera of 193 from healthy pregnant women were screened for HBsAg. Sera with HBsAg-negative and anti-HBc-positive were tested for anti-HBs, then HBV DNA were examined by polymerase chain reaction and direct-sequencing. Of 193 sera, 141 were HBsAg-negative and anti-HBc positive. In 50 of 141 sera, HBV DNA was detected in 9 (18%). Eleven of 50 were positive for anti-HBs. The HBV/B3-adw2 was predominant in 7 (77.7%), followed by HBV/B9-ayw1 in 1 (11.1%), and HBV/C7-adrq+ in 1 (11.1%). Three isolates had amino acid mutations (Q129H, T131N, M133S, T140I, T126I) in the �a� determinant region. The prevalence of OBI in pregnant women from Surabaya is quite high, but still in accordance with those among the general population in Asia. HBsAg detection failure in this study might be due to mutations in the �a� determinant region and/or the low-level of HBV viral load. Additional testing of pregnant women with an anti-HBc test and for there with anti-HBc-positive the HBV DNA detection could be considered.

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