Lynda Rossyanti, NIM011414253004 (2016) PROFIL GEN PLASMODIUM FALCIPARUM CHLOROQUINE RESISTANCE TRANSPORTER KODON 72-76 DAN MULTIDRUG RESISTANCE 1 KODON 86 DI KABUPATEN BARITO UTARA KALIMANTAN TENGAH. Thesis thesis, Universitas Airlangga.

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Abstract

People living at remote areas are common consume anti malarial drugs by themselves especially chloroquine. To aim to identify the Plasmodium falciparum resistance to chloroquine, Plasmodium falciparum chloroquine resistance transporter in codon 72-76 and Plasmodium falciparum multidrug resistance transporter 1 in codon 86 were examined. A cross sectional study was conducted in Sei Rahayu 1 sub district, North Barito, Middle Kalimantan in April 2016. Plasmodium parasites were identified by microscopic examination and nested PCR method. The polymorphism in codon 72-76 of PfCRT was identified by DNA sequencing and in codon of 86 of PfMDR1 was detected by PCR-RFLP. In total 16 of 219 samples (7,3%) were infected with Plasmodium falciparum by microscopic examination and 13 of 16 samples (81,25%) were positive P. falciparum by nested PCR method. Two types of PfCRT72-76 haplotypes were detected from 4 P. falciparum isolates that were CVMNK (2/4) and CVIET (2/4) (Underline word is an amino acid substitution). All P. falciparum isolates showed the wild type of PfMDR186. Five stalls (5/6, 83.3%) sell chloroquine. It suggested that the use of chloroquine by people and the use of ACT may involve in the formation process of these PfCRT72-76. haplotypes in P. falciparum isolates in Sei Rahayu I. Thus, molecular marker for anti malatial drugs resistance in P. falciparum and anti malarial drugs distribution should be regularly monitored in order to reduce mutant parasites and their spreading, particularly in Sei Rahayu 1, North Barito district, Middle Kalimantan.

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