Dwi Kusuma Wahyuni, S.Si., M.Si. and DEDY PRASETYO, 091044001 and Sucipto Hariyanto, Dr. DEA (2014) Perkembangan Kultur Daun Aglaonema sp. dengan Perlakuan Kombinasi Zat Pengatur Tumbuh NAA dan 2,4-D dengan BAP (The Leaf Culture Development of Aglaonema sp. Treated by Combination of NAA, 2,4-D and BAP as Growth Regulators). Jurnal Bios Logos, 4 (1). ISSN 2088-9569

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Abstract

The objective of this study were to determine the effect of combination of NAA and 2,4-D with BAP toward leaf culture development and to know the best concentration of growth regulator substance to induce callus on leaf explant of three Aglaonema cultivars. The cultivars were Dynamic Ruby, Snow White, and Siam Aurora. Leaf explants were cultured on solid MS medium with addition of various concentration combination of NAA and 2,4-D with BAP. Result of eight week observation gave significantly effect to changes shape leaf. Leaf blade was curved or rolled up, swelled, leaf color became pale strands, and formed callus, but the only cultivar which formed callus was Aglaonema sp. cv. Dynamic Ruby. The conclusion of this study was combination of growth regulator substance 0,1 ppm 2,4-D and 1 ppm BAP are the appropriate combination on callus inducing for Aglaonema sp. cv. Dynamic Ruby.

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