RENNA INTAN RUMANIKASIH, 081211431006 (2016) INDUKSI POLISAKARIDA KRESTIN DARI EKSTRAK Coriolus versicolor TERHADAP KONSENTASI IL-23 DAN JUMLAH NEKROSIS HEPATOSIT Mus musculus YANG DIINFEKSI Klebsiella pneumoniae. Skripsi thesis, Universitas Airlangga.
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Abstract
This research was aimed to know the induction of polysaccharide krestin from Coriolus versicolor extract on IL-23 concentration and hepatocyte necrosis of mice infected by K. pneumoniae. Thirty adult female mice of Mus musculus strain Balb/C, age 8 – 10 weeks and weight 30 – 40 g, were sacrificed. Mice were divided into six treatment groups, that were: group K was control; group K+ was positive control; group K- was negative control; group P1 was added PSK before being exposed by K. pneumoniae; group P2 was added PSK after being exposed by K. pneumoniae; group P3 was added PSK before and after being exposed by K. pneumoniae. Every treatment had four replications. PSK was added 100 mg/kg body weight in dose while K. pneumoniae were infected in 1,5 x 108 cell bactery. IL-23 concentration was determined by Mouse ELISA Kit and hepatocyte necrosis were made to histology slide. Data of IL-23 concentration were analyzed by Brown Forsythe test and Games Howell test (α = 0,05). Data of hepatocyte necrosis concentration were analyzed by One Way ANOVA and Duncan test (α = 0,05). The result of this research showed that P3 had the highest IL-23 concentration (141,5 pg/mL) and K- had the lowest IL-23 concentration (141,5 pg/mL). IL-23 concentrations of K, K+, P1 and P2 were 77,98 pg/mL, 88,81 pg/mL, 72,24 pg/mL and 64,28 pg/mL respectively. Meanwhile, the highest average amount of hepatocyte necrosis was K- (14,56 cell/cm2) and the lowest average amount of hepatocyte necrosis was K (11,42 cell/cm2). Average amount of hepatocyte necrosis in K+, P1, P2, and P3 were 13,57 cell/cm2, 13,75 cell/cm2, 14,29 cell/cm2 and 12,97 cell/cm2 respectively. The conclusion of this research was that PSK could increase IL-23 concentration and decrease hepatocyte necrosis of mice with before and after more infected K. pneumoniae as potentialtime.
Item Type: | Thesis (Skripsi) | ||||||
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Additional Information: | KKC KK MPB 56-16 Rum i | ||||||
Uncontrolled Keywords: | Hepatocyte necrosis, IL-23, K. pneumoniae | ||||||
Subjects: | Q Science > QR Microbiology > QR1-502 Microbiology | ||||||
Divisions: | 08. Fakultas Sains dan Teknologi > Biologi | ||||||
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Depositing User: | Mrs. Djuwarnik Djuwey | ||||||
Date Deposited: | 09 Feb 2017 18:18 | ||||||
Last Modified: | 18 Jun 2017 18:02 | ||||||
URI: | http://repository.unair.ac.id/id/eprint/52615 | ||||||
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