MOHAMMAD HARTANTO YUSUFA, 061414253012 (2016) OPTIMASI MEDIA PRODUKSI DAN UJI AKTIVITAS ANTIMIKROBA CRUDE LIPOPEPTIDA BIOSURFAKTAN Bacillus subtilis ASAL LIMBAH CAIR RUMAH POTONG AYAM TRADISIONAL KOTA MALANG PENELITIAN EKSPLORATIF LABORATORIS. Thesis thesis, Universitas Airlangga.

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Abstract

This research aimed to discover production optimization and antimicrobial activity of Bacillus subtilis crude lipopeptide biosurfactant from Poultry Slaughterhouse’s waste. Media optimization for biosurfactant production used basal media and carbon source combination. Media production used Nutrient Glucose Broth (NGB) 5 %, and Nutrient Molasses Broth (NMB) 5%, 10%, and 15%. Drop collapse were used as the biosurfactant detection method. Acidity technique was used to isolate crude lipopeptide biosurfactant. Antimicrobial activity against gram positive (S. aureus) and negative bacteria (E. coli) was examined using diffusion disc method in order to investigate the inhibition of each bacteria. Temperature treatment was used to know effect on crude lipopeptide biosurfactant bacteriostatic activity against S. aureus. NMB 5% was optimum media for B. subtilis biosurfactant production. The crude lipopeptide biosurfactant has bacteriostatic activity against S. aureus and E. coli. The crude lipopeptide biosurfactant was stable on temperature treatment. Key words : Crude lipopeptide biosurfactant, Media, Antimicrobial

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