Nike Hendrijantini, NIDN. 0006105908 and Utari Kresnoadi, NIDN. 0011015403 and Sherman Salim, NIDN. 8833370018 and Bambang Agustono, NIDN. 0027086304 and Endang Retnowati, NIDN. 071608640 and Iwan Syahrial and Pungky Mulawardhana, NIDN. 0003068107 and Manggala Pasca Wardhana, NIDN. 0020118404 and Coen Pramono, NIDN. 0010025405 and Fedik Abdul Rantam, NIDN. 0010035907 (2015) Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells(hUCMSCs) with Gelatin Solvent. Journal Biomedical Science and Engineering, 8 (7). pp. 420-428. ISSN 1937-6871

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Abstract

The human umbilical cord is a source of numerous Mesenchymal Stem Cells (MSCs), making it as a potential source of allogeneic multipotent cell for bone tissue engineering. The aims of this study were to find: 1) Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) phenotypic characterization, 2) The in-vitro osteogenic differentiation potential of hUCMSCs, 3) The cytotoxicity of gelatin solvent to hUCMSCs using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide(MTT) assay. As a result, through characterization of hUCMSCs, the majority of target cells expressed specific MSCs markers, Cellular Differentiation (CD)73, smaller number of subpopulation expressed CD90 with only minimal subpopulation expressed CD105 and all negative MSCs markers. Osteoblastic differentiation was found in a significantly high number of cells when in vitro osteogenic differentiation of hUCMSCs with Alizarin Red staining was done. Biocompatibility analysis using the MTT assay showed that gelatin solvent and Alpha modification of minimum essential

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