Prasetyo, R. Heru and Safitri, Erma (2016) Effects of Honey to mobilize endogenous stem cells in efforts intestinal and ovarian tissue regeneration in rats with protein energy malnutrition. Asian Pacific Journal of Reproduction, 5 (3). pp. 198-203. ISSN 2305-0500, 2305-0519

	Text (Peerreview) Peerreview3.Effect of Honey.pdf Restricted to Registered users only Download (809kB) | Request a copy
Preview	Text (ARTIKEL JURNAL) karil3. effect of honey oke-min.pdf Download (414kB) | Preview

Abstract

Objective: Auto-regeneration of the intestinal and ovarian tissue are experiencing degenerative due to protein energy malnutrition (PEM) through of auto-mobilization, increase of immune response and differentiation of endogenous stem cells. Methods: Female rat model of PEM obtained through fasting meals for 5 d and only to drink, causing malnutrition and damage of the intestinal and ovarian tissue. Furthermore, the therapy of honey with a dose of 30% water (T1) and 50% water (T2), respectively for 5 d and compared with the positive control, were fasted without being given honey (T0+) and negative control, not fasted and without being given honey (T0 − ). Observations on: auto-mobilization, increased immune response, differentiation of stem cells, and regen- eration of the intestinal and ovarian tissue with HE staining. Results: Auto-mobilization of stem cells based on the expression of CD34+ and CD45+, which is a marker of endogenous stem cells (hematopoietic stem cells/HSCs). Increased immune response is based on Hsp70 expression and PGE2 in intestinal tissue. Differ- entiation of stem cells into progenitor cells that expected based expression of growth differentiation factor-9 (GDF-9) by immunohistochemistry in ovarian tissue. Conclusions: Expression of CD34+ and CD45+, which signi fi cantly different in treat- ment 2 (2). Furthermore, increase of immune response (decrease Hsp70 expression and increased PGE2) in intestinal tissue. Increased immune response causes expression of GDF-9 in ovarian tissue. Decreased of Hsp70 expression, increased PGE2 and increased GDF-9 followed the process of regeneration of the intestinal and ovarian tissue.

Actions (login required)

View Item