Mahaputra, Laba and Koesdarto, Setiawan and Agus Hermadi, Herry and Mulyati, Sri (2012) Biocompability of Low Dose of Human Menopause Gonadotropin (hMG) and Deglycosilation of Human Menopause Gonadotropine (dGhMG) Against Bovine Eggs. Jurnal Biosains Pascasarjana, 14 (2). pp. 93-96. ISSN 1412-1433

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Abstract

No any researcher yet reported by adding gonadotropin mainly either hMG or dGhMG less than 1 ug/ml into media succesfully obtainproportional amount fertilized eggs in invitro fertilization technique (IVF), moreover this dosis twice and four times decreased from1000 ng. The main purpose of this study was to evaluate biocompatibility of low dosis of hMG and dGhMG as additive in mediamaturation during in-vitro maturation (IVM) to amount of fertilized egg obtained following 48 hrs of in-vitro insemination (IVI). Allthose inclusive criteria oocytes that collected in laboratorium invitro Lab. obstetrics Department of Reproduction, Vet medicine,Unairrandomized into sub-group (1, 2, 3, and 4) and also control group. Sub-group 1, 2, 3, 4 and control group Containing with TC199 +10% bovine calf serum. Sub-group 1 added with hMG 250 ng/ml and 500 ng/ml for sub-group 2. Sub-group 3 and 4 added with 250ng/ml and 500ng/ml dGhMG respectively . all these immature oocytes were transfer into those each drop 50ul media in 35 mm disposiblepetridisk and contained each drop 5–7 oocytes then covered in with mineral oil and polyesterene plastic petridisk cover then incubatedfor 24 hrs in 5% CO2 incubator. Next morning after oocytes washed in warm Media oocyte washing solution (OWS) and once usingEarle’s balanced salt solution (EBSS) all predicted mature oocytes transfered into centripetal side of Rosset patern made using EBSSmedia 10ul each , that connected each other to centre of centripetal side 50ul .This centre side of centripetal was transfered with 30 ulsemen have been followed and washed twice then swim-up process then kept in 5% CO2, 38.5° C for 24 hrs. Observation performed2 × 24 hrs after all oocytes transfer into maturation media 24 hrs following IVI . All sub-group showed increasing amount of fertilizedeggs higher than 75% and much higher compared to control group that only 38.1%, but among subgroup was not much differed,however the other hand found highest fertilized eggs/embryo was 87.5% in sub-group 4. Wishing to reach more fertilized eggs in IVF,gonatropine hormone absolutely should be added in basically maturation media. hMG , and dGhMG in bovine eggs, of IVF

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