Imelda Kristina Sutrisno and Ira Arundina and Agung Sosiawan (2013) Identifikasi bite marks dengan ekstraksi DNA metode Chelex. Dental Jurnal (Majalah Kedokteran Gigi), 46 (2). pp. 107-112. ISSN 1978 - 3728
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Abstract
abstract Background: In the case of crime often encountered evidence in bite marks form that was found on the victim’s body. Generally, bitemarks identification use standard techniques that compare the interpretation picture with the tooth model of suspected person. However, sometimes the techniques do not obtain accurate results. Therefore another technique is needed to support the identification process,such as DNA analysis that use the remaining epithelium attached in saliva to identify the DNA of the suspected person. In this processes a limited DNA material could be met, not only less in quantity but also less in quality. Chelex known as one of an effective DNA extraction method in DNA forensic case is needed to overcome this problem. Purpose: The study was aimed to examine the use of Chelex as DNA extraction method on a bitemarks sample models. Methods: The blood and bitemarks of 5 persons with were taken. The DNA of each subject was exctracted with Chelex and quantified the quantity with UV Spechtrophotometer. The DNA results was amplified by PCR at locus vWA and TH01 then vizualised by electrophoresis. Results: The electrophoresis’s results showed band at locus vWA and TH01 for blood sample and bite marks with no significant differences. Conclusion: The study showed that Chelex method could be use to extract DNA from bitemarks. Key words: Bite marks, Chelex, locus vWA and TH01
| Item Type: | Article | ||||||||
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| Uncontrolled Keywords: | Bite marks, Chelex, locus vWA and TH01 | ||||||||
| Subjects: | L Education Q Science R Medicine > RK Dentistry |
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| Divisions: | 02. Fakultas Kedokteran Gigi Peer Review |
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| Depositing User: | Rudy Febiyanto | ||||||||
| Date Deposited: | 05 Apr 2017 21:15 | ||||||||
| Last Modified: | 05 Apr 2017 21:15 | ||||||||
| URI: | http://repository.unair.ac.id/id/eprint/55189 | ||||||||
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