SLAMET NOVERIANTO, 059912164
(2004)
VALIDASI METODE KLT -DENSITOMETRI UNTUK PENETAPAN KADAR ASAM OKSOLINAT DALAM UDANG.
Skripsi thesis, Universitas Airlangga.
Abstract
A simple TLC densitometric method has been developed for the determination of oxolinic acid residue in shrimps. The method involves use of silica gel OF 254 nm after being impregnated with 5% EDT A, and chloroform-aceton (10:1, v/v) as a mobile phase. The separated bands were detected at A 260 nm. The respond was found to be linearity dependent on amount of oxolinic acid between
62.50 ngl5}.11 and 312.88 ngl5}.11 (r = 0.9943, Y = 22.475 X + 411.58, Vxo 6.59%). The method was validated to determine the limit of detection, limit of quantitation, accuracy and precision. The results showed that the limit of detection and limit of quantitation value are 7.81 ngl5}.11 and 26.04 ngl5}.11, where as the accuracy and precision are 63.98% and 7.79%, respectively.
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