Dewi Melani Hariyadi and Tristiana Erawati (2015) Physical characteristics and release study of ovalbumin from alginate microspheres by different concentration of alginate and BaCl2 using aerosolization technique. In: The 1st International Conference on Pharmaceutics and Pharmaceuticals Sciences Proceedings. Faculty of Pharmacy Universitas Airlangga, Surabaya, pp. 46-49. ISBN 978-602-72333-0-0

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Abstract

Microsphere formulations have been widely used for oral applications. The aim of this research was to study physical characteristics and release study of ovalbumin from alginate microspheres prepared by different concentration of aiginate polymer and BaCI2. This research used concentrations of BaCl2 of 0,5M and 0,75M and concentrations of alginate of 2,5% w/v and 3,5% w/v. Ionotropic gelation using aerosolisation technique was applied in this study. All ovalbumin - loaded alginate microspheres were characterized in terms of size, morphology, protein loading, encapsulation efficiency, yield, and release profile of ovalbumin. In vitro release study was conducted in the simulated gastric fluid (HCI pH 1.2) and simulatedintestinal fluid (PBS pH 7,4) at temperature 370C. Results showed spherical and smooth microspheres were produced. In addition, smaller particle size of less than 8 pm was produced by increasing alginate and BaCl2 concentration. Afactorial design ANOVA and one way ANOVA were used for statistical analysis at a 95% confidence interval. No significant effect was shown by increasing alginate and BaCI2 concentration on the protein loading, encapsulation efficiency, and yield. No significant differences of ovalbumin release were found when increasing concentration of BaCI2 from 0,5M to 0,75M, however ovalbumin release decreased by increasing alginate concentration and slower release in HCI pH 1,2 during 2 hours followed by complete release in PBS pH 7,4after 17 hours

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