ATIKA CANDRA BINARTI, 051211131010 (2016) PENGARUH SINAR MATAHARI TERHADAP KADAR EGCG SALAH SATU PRODUK MINUMAN TEH HIJAU KEMASAN BOTOL PLASTIK. Skripsi thesis, Universitas Airlangga.

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Abstract

THE SUNLIGHT EFFECT ON EGCG CONTENT IN ONE OF GREEN TEA BEVERAGE PRODUCT PACKAGED IN PLASCTIC BOTTLES Atika Candra Binarti Epigallocatechin gallate (EGCG) is a green tea catechin with potential health benefits, such as anti-oxidant, anti-carcinogenic and antiinflammatory effects. In general, EGCG is highly susceptible to degradation, therefore presenting stability problems. The research was focused on the study of the sunlight effect on EGCG content during storage for 12 weeks. The samples were stored in 2 different condition, protected from the sun and exposed to the sun for 3 hours. The samples would be sampled at week-0, week-4, week-8 and week-12. The HPLC condition was as follows : RP C- 18 μbondapak 10μm, 3.9 x 300 mm used as column, the mobile phase was metanol : water : acetic acid (2%) = 20 : 75 : 5 (v/v/v), flow rate 1.0 ml/min, and detection monitored at 274.0 nm with spectrophotometry UV-Vis. From this study, there was a different level of EGCG in green tea product packaged in plastic bottles. The levels of EGCG in exposed to the sun green tea beverage product decreased by 52.66% while for green tea beverage product stored at room temperature decreased by 26.04%. Using paired-sampels ttest, it was proven that sun light could decrease the levels of EGCG in green tea product packaged in plastic bottles faster. Keywords : Green tea, plastic bottles, sun light, room storage conditions, Epigallocatechin gallate (EGCG), HPLC.

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