Achmad, Fuad Hafid and Lidya, Tumewu and Evhy, Apryani and Mei, Ria Santi and Tutik, Sri Wahyuni and Adita, Ayu Permanasari and Myrna, Adianti and Chie, Aoki and Aty, Widyawaruyanti and Maria, Inge Lusida and Soetjipto, Soetjipto and Hak, Hotta (2016) AntiHepatitis C Virus Activity of Alectryon serratus Leaves Extract. Procedia Chemistry, 18. pp. 169-173. ISSN 18766196

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Abstract

Hepatitis C Virus (HCV) has infected approximately 2-3% (130-170 million) of the world's population. No vaccine is available to prevent HCV infection. Investigation of anti-HCV agent is thus deemed necessary. Various plants have been explored for their anti-HCV activity. A. serratus is a member of Sapindaceae family, which fruit and seed were traditionally used as insecticide. Anti-HCV activity tested on A.serratus leaves extract has been done. The result showed that leaves extract exhibited anti-HCV with IC50 value of 14.9 μg/ml and 9.8 μg/ml against HCV J6/JFH1 and JFH1a, respectively. The cytotoxicity assay results showed that A.serratus leaves extract was not toxic and has CC50 >100 μg/ml. Mode of action experiment results suggested that A.serratus extract inhibited HCV at the post-entry step. Further fractionation of leaves extract by open column chromatography resulted in 4 fractions. Only Fraction 1 (AP-5F.1) exhibited anti-HCV with IC50 value of 1.2 μg/ml against HCV JFH1a. Separation of AP-5F.1 by open column chromatography resulted in 15 fractions. Fraction number 13 (AP-5F.1.13) exhibited anti-HCV with IC50 value of 0.43 μg/ml against HCV JFH1a. Separation of AP-5F.1.13 by semi preparative-HPLC resulted in isolate identified by TLC and LC-MS method as chlorophyll derivate. There was a possibility that chlorophyll derivate has participated in performing the anti-HCV activity of fractions and extract besides the other compounds contained. In this study, we concluded that A. serratus leaves extract, AP-5F.1, and AP-5F.1.13 exhibited anti-HCV activity against JFH1a virus.

Item Type:	Article
Uncontrolled Keywords:	Alectryon serratus; leaves extract; antiHepatitis C Virus
Subjects:	Q Science > QR Microbiology > QR355 Virology R Medicine R Medicine > RS Pharmacy and materia medica R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
Divisions:	05. Fakultas Farmasi > Farmakognosi Fitokimia
Creators:	Creators NIM Achmad, Fuad Hafid achmadfuad@ff.unair.ac.id Lidya, Tumewu UNSPECIFIED Evhy, Apryani UNSPECIFIED Mei, Ria Santi UNSPECIFIED Tutik, Sri Wahyuni UNSPECIFIED Adita, Ayu Permanasari UNSPECIFIED Myrna, Adianti UNSPECIFIED Chie, Aoki UNSPECIFIED Aty, Widyawaruyanti aty-w@ff.unair.ac.id Maria, Inge Lusida UNSPECIFIED Soetjipto, Soetjipto UNSPECIFIED Hak, Hotta UNSPECIFIED
Depositing User:	Mr M. Fuad Sofyan
Date Deposited:	08 May 2017 21:40
Last Modified:	08 May 2017 21:40
URI:	http://repository.unair.ac.id/id/eprint/56584
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