Winarko, Michael Natanael and Sosiawan, Agung and Eleonora, Sophia (2013) Keberhasilan Metode Ekstraksi FTATM Paper pada pemeriksaan DNA Bitemark Lokus TH01,vWA di Bidang Kedokteran Gigi Forensik. Oral Biology Dental Journal, Vol.5 (No.1). January- June. ISSN 979-8814

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Abstract

ABSTRACT Backgroud. Forensic case often encountered bitemark evidence that was found on the victim’s or perpetrator’s body. Bitemark can be compared with the tooth model of suspected person physically. However, this technique may cause subjective interpretations. Therefore, an alternative technique to identify the bitemark case was used. The biological evidence from bitemark may be taken by irrigating the bitemark to collect the saliva and buccal epithelium deposited during biting so that the DNA from the suspected person can be identified. In the fact DNA material that has been found was very limited and might be degraded, less both in quantity and quality. Due to that, an effective method to extract the DNA material is needed. Purpose. Proving the success of DNA extraction with FTATMPaper method through PCR-Short Tandem Repeat (STR) on TH01,vWA loci. Method. Blood and bitemark samples were taken from 5 persons, each sample was exctracted with FTATMPaper method. When a sample is applied to the FTATMPaper the cell membranes are lysed, the nucleic acids are released and the DNA is entrapped within the fibres of the cards’ matrix. Purification/extraction of the DNA is simple (only requiring a series of wash steps). This means that DNA quantitation need not and cannot be measured/performed for samples extracted from FTATMPaper. DNA samples were amplified with PCR which vWA and TH01 loci were as the target. Visualizing band appearances were using 6% polyacrylamide gel electrophoresis. Result. Electrophoresis visualisations show band appearances of vWA and TH01 loci from blood and bitemark samples which mean the FTATMPaper method can be used for bitemark. Conclusion. It was proven that FTATMPaper extraction can be used effectively to extract DNA from bitemark. Keywords: bitemark, FTATMPaper, vWA, and TH01 loci

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