EMBRYO COLLECTION TOWARD DIFFERENT DOSES OF PMSG IN RATS Repository

EMBRYO COLLECTION TOWARD DIFFERENT DOSES OF PMSG IN RATS

Bambang Poernomo S. (2013) EMBRYO COLLECTION TOWARD DIFFERENT DOSES OF PMSG IN RATS. PROCEEDING International Seminar : THE ROLE OF VETERINARY SCIENCE TO SUPPORT MILIENNIUM DEVELOPMENT GOALS AND THE 12th ASIAN ASSOCIATION OF VETERINARY SCHOOLS CONGRESS JW MARRIOTT HOTEL, SURABAYA-INDONESIA 4th-6th SEPTEMBER 2013 . Faculty of Veterinary Medicine Universitas Airlangga, Surabaya. ISBN 978-502-70438-0-0

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Abstract

Technique for induction of super ovulation in livestock industry has been applied, particuiarly when large numbers of embryos are required for embryo transfer' The purpose of the research was collected of the embryos among group which it treated different doses of PMSG. All population of female rats were divided into three groups consist of 10 animals each group. Each animal were iniected intra peritoneal at volume 0.1 cc. Doses of PMSG (lntergonan@ - Vemie) were 0 IU, 10 lU, and 20 IU, respectively, on the day minus two. Therefore, all animals were iniected intra peritoneal on day zero through 10 lU HCG (Chorulon@ - Interved). On the day four, all mated females were sacrificed and the uterus was taken out. A blunt 21 gauge needle was inserted through the cervic into the lumen of uteri. Then the Fallopian tubes were cut off. Embryo collection were recovered through flushing 1cc Phosphate Buffer Solution (PBSI add with 200lo Fetal Calf Serum (FCSI supplemented. The treated group administered dose 20 lU PMSG tend to increased number of eggs super ovulated On the similar way, the treated group administered dose 10 lU PMSG tend to increased too. However, there was no significantly difference between both 10 IU PMSG group and 20 lu PMSG according to the number of mated rats and average of embryos each rats on P>0.01. On the treated rats in both PMSG doses 10 IU and 20 IU, a number of embryos were found degenerated at the time of observation. The degenerated ovum was shown uncompacted or Ioss blastomer cells in inside ofthe zona pellucida. These undoubtedly caused by the high degree of fertilization failure, This research suggested that the dose of PMSG play the important role as a source of variation in embryo collection.

Item Type:

Book

Uncontrolled Keywords:

Embryo Collection, PMSG, Morula, Blastula

Subjects:

R Medicine > RG Gynecology and obstetrics > RG1-991 Gynecology and obstetrics > RG500-991 Obstetrics > RG600-650 The embryo and fetus
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology

Divisions:

06. Fakultas Kedokteran Hewan
Peer Review

Creators: