VIVA AYU KARTIKA, 061211132115 (2016) DETEKSI ANTIBODI DALAM SERUM AYAM YANG DIINFEKSI LARVA STADIUM KEDUA (L2) Toxocara cati DENGAN DOSIS BERBEDA MENGGUNAKAN TEKNIK Indirect-ELISA. Skripsi thesis, Universitas Airlangga.

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Abstract

The purpose of this research is to analyze the differences of Optical Density (OD) value between antigens of Toxocara cati with serum anti-L2 Toxocara cati in chicken by indirect-ELISA technique. The OD value can be used as a development toxocariasis diagnostic. The First step was infected the chickens with L2 Toxocara cati with doses 10, 100, 1000, and 3000. The Second, taking the chicken's blood from vena brachialis at 28 days post infection. The third step was to make Toxocara cati homogenates obtained by Toxocara cati mashed, then suspended in 1 ml of PBS and centrifuge. Then, doing diagnostic test by indirect ELISA with 1:50, 1:100, 1:200 diluton. The result of indirect-ELISA was measured using ELISA reader with 405 nm wave-length. The average of OD values for each doses in 1:200 dilution obtained through this measurement was 0.287, 1.027, 1.044, 0.876, and 1.025. The result of statistical analysis using F (one way ANOVA) 1% test indicates significant difference (p< 0.01). Based on Duncan 5% test advanced analysis, there was no significant difference (p> 0.05).

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